Thermo Fisher Scientific Offers 30 New ChIP-validated Antibodies

ROCKFORD, Ill. (Sept. 2, 2010) – Thermo Fisher Scientific, the world leader in serving science, today introduced the Thermo Scientific Pierce Chromatin Immunoprecipitation (ChIP)-validated Antibodies. With Pierce® ChIP-validated Antibodies, researchers can obtain results more quickly by avoiding the testing of multiple antibodies to determine their suitability in ChIP assays., 

The Pierce ChIP-validated Antibodies are a perfect complement to the recently launched Thermo Scientific Pierce Agarose ChIP Kit. Thirty ChIP-validated antibodies are available to common targets, including numerous histone and transcription factor proteins.

For more information on Pierce ChIP-validated Antibodies, please call +1-815-968-0747 or visit www.thermoscientific.com/pierce.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of more than $10 billion, we have approximately 35,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications. Visit www.thermofisher.com.

Thermo Fisher Scientific Offers New Protein Labeling Tools for Mass Spectrometric Analysis

ROCKFORD, Ill. (June 25, 2010) — Thermo Fisher Scientific, the world leader in serving science, today announced the expansion of its isobaric tandem mass tags product line with the new Thermo Scientific Cysteine-Reactive Tandem Mass Tag (cysTMT) Reagents. The cysTMT® Reagents enable protein identification and quantitation through the selective labeling of cysteine-containing peptides from complex biological samples. The labeling system can be used in multiplex analysis of up to six different samples concurrently in an experiment.

The cysTMT Reagents label only free sulfhydryl groups on cysteine residues, in contrast to the amine-reactive TMT® Reagents. Labeled peptides are then enriched using the Thermo Scientific Immobilized Anti-TMT Antibody Resin. The cysTMT Reagents and Anti-TMT Resin are effective for reducing sample complexity, improving dynamic range and studying cysteine modifications. The cysTMTzero and cysTMTsixplex Reagents are used as isotopic “light” and “heavy” duplex tags for quantitation at the MS stage. These tags enable quantitation of protein expression changes in cell and tissue samples that might not be amenable to metabolic isotopic labeling strategies (e.g., SILAC). 

For more information on the cysTMT Reagents and Anti-TMT Resin, visit www.thermoscientific.com/pierce or call +1-815-968-0747.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of more than $10 billion, we have approximately 35,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications. Visit www.thermofisher.com.

Thermo Fisher Scientific Introduces New Tools for Phosphopeptide Enrichment and Mass Spectrometric Analysis

ROCKFORD, Ill. (June 22, 1010) — Thermo Fisher Scientific, the world leader in serving science, today announced the availability of the Thermo Scientific Pierce Fe-NTA Phosphopeptide Enrichment Kit. With the Pierce® Fe-NTA Phosphopeptide Enrichment Kit, scientists can process protein digests and strong cation exchange fractions to selectively recover phosphopeptides for mass spectrometric analysis in less than 30 minutes.

The Pierce Fe-NTA Phosphopeptide Enrichment Kit supplies high-capacity Fe-NTA spin columns and optimized buffer for analyzing 30 protein digests. The phosphopeptide-specific resin in each spin column will sufficiently enrich up to 150 micrograms of phosphopeptides. The Pierce Fe-NTA Spin Columns complement other Thermo Scientific lysis, reduction, alkylation and digestion reagents and graphite spin columns to provide a complete workflow for phosphopeptide enrichment.

For more information on the Pierce Fe-NTA Phosphopeptide Enrichment Kit, visit www.thermoscientific.com/pierce or call +1-815-968-0747.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of more than $10 billion, we have approximately 35,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Our products and services help accelerate the pace of scientific discovery, and solve analytical challenges ranging from complex research to routine testing to field applications. Visit www.thermofisher.com.

Thermo Fisher Scientific Introduces Two Novel Kits for the Non-radioactive Study of RNA:Protein Interactions

ROCKFORD, Ill. (June 10, 2010) — Thermo Fisher Scientific today announced the availability of the Thermo Scientific LightShift RNA EMSA Kit and the Thermo Scientific Pierce RNA 3' End Biotinylation Kit for research into RNA:protein interactions using non-radioactive methods.

The LightShift® RNA EMSA Kit is the first commercially available kit for research using RNA electrophoretic mobility shift assay (EMSA). The kit uses a biotinylated RNA fragment to probe protein samples. RNA:protein complexes are then monitored by changes in electrophoretic migration patterns. To detect these changes, the kit uses chemiluminescent detection methods that provide comparable sensitivity to radioactive detection. The non-radioactive detection method also produces X-ray results in one to five minutes, a vast improvement over 16-hour exposures typically needed for radioactive systems.

The easy-to-use LightShift RNA EMSA Kit includes all reagents required to set up and optimize protein:RNA binding conditions, a positive control for protein:RNA interactions and reagents for chemiluminescent detection of the nucleic acid interaction. The system is compatible with biotinylated RNA probes produced by a variety of methods, including the new Pierce® RNA 3’ End Biotinylation Kit.

The patent pending Pierce RNA 3′ End Biotinylation Kit provides the reagents to produce bionylated RNA probes for use in chemiluminescent detection methods. The labeling reaction uses an enzymatic approach to ligate a single biotinylated nucleotide to the 3′ end of an RNA strand that is 22 to 450 nucleotides in length. The reaction can be completed in 30 minutes to two hours. The resultant biotinylated RNA probes are produced at a fraction of the cost of synthetic biotinylated RNA probes.

The method used by the Pierce RNA 3’ End Biotinylation Kit preserves the RNA probe structure, which is be important in protein:RNA interaction experiments. The RNA probes produced by the kit can be used with the LightShift RNA EMSA Kit or to perform RNA pull-down assays, Northern blots or miRNA profile studies. The complete kit contains an unlabeled RNA strand to use as a positive control, a biotinylated-RNA probe standard, RNase inhibitor, glycogen and ligation-enhancing reagents.

For more information on either the LightShift RNA EMSA Kit or the Pierce RNA 3’ End Biotinylation Kit, visit www.thermoscientific.com/pierce or call +1-815-968-0747.

Thermo Scientific is part of Thermo Fisher Scientific, the world leader in serving science.